L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is...

β-Arrestin GPCR cells are engineered to co-the ProLink (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent...

Magnesium Assay Kit is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (?max450nm) product whose formation is proportional to Mg2+ concentration. The linear...

β-Arrestin GPCR cells are engineered to co-the ProLink (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent...

β-Arrestin GPCR cells are engineered to co-the ProLink (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent...

NHR Protein Interaction assays monitor the interaction of activated NHRs with Steroid Receptor Co-activator Peptides (SRCPs) using Enzyme Fragment Complementation. The cells are engineered to two complementary fragments of β-galactosidase: the smaller ProLink (PK) fragment is fused to the NHR target of interest, and the larger Enzyme Acceptor...

NHR Protein Interaction assays monitor the interaction of activated NHRs with Steroid Receptor Co-activator Peptides (SRCPs) using Enzyme Fragment Complementation. The cells are engineered to two complementary fragments of β-galactosidase: the smaller ProLink (PK) fragment is fused to the NHR target of interest, and the larger Enzyme Acceptor...

Renilla Luciferase Assay Kit is designed to provide a simple and sensitive method of detecting Renilla luciferase. Through the use of a specially designed buffer formulation, this assay kit yields reliable, linear results with minimal autoluminescence background and superior...

This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. (200 assays) Simple procedure; takes ~ 20-30 minutesFast and convenientThe assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple...

The Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working...